Effects on Soil Bacterial Organisms in an Alfalfa Monocropping System after Corn Insertion and Nitrogen Fertilization

Alfalfa (Medicago sativa L.) and corn (Zea mays are common forage plants for feeding livestock, their effects on soil bacterial organisms have been extensively studied. However, there is little comprehensive research function in a long-term alfalfa monocropping system after insertion fertilization. The of alfalfa–corn rotation (AF: monocropping, RA: rotation) nitrogen fertilization (RA0 RA15) were investigated field experiment. results showed that significantly increased the aboveground biomass (AGB) nitrate nitrogen, decreased (NO3−-N) (p < 0.05). In addition, community structure among treatments was changed by rotational RA diversity compared with AF 0.01), most communities phyla Actinobacterota Proteobacteria. had lower relative abundance than system. prediction found carbon cycle processes more active those AF. RDA analysis revealed NO3−-N pH main environmental factors affecting